20th Annual UltraScan Analytical Ultracentrifugation Workshop 2023

August 21-25, 2023, in Lethbridge, Alberta, Canada.

Sponsored by:
AUC Solutions and the Canadian Center for Hydrodynamics


Solution Studies of Biological Macromolecules:
From Experimental Design to Data Analysis - UltraScan-III Workshop


Instructor:
Dr. Borries Demeler, Professor, Canada 150 Research Chair for Biophysics, University of Lethbridge
Morning Session (9:00 am - 12:30 pm)
ROOM SA6008
Afternoon Session (1:30 pm - 5:00 pm)
ROOM SA6008
Session 1: Monday, 8/21/23 - AUC 101: Instrument Basics
  • Cell Assembly
  • The Optima AUC
  • Rotor setup and cell alignment
  • UltraScan overview and Registration
  • UltraScan-in-a-Box setup, access via ssh and vnc
  • UltraScan setup for a local install
  • Accessing data on your Optima AUC from UltraScan
Session 2: Monday, 8/21/23 - AUC 102: UltraScan Basics
  • AUC and Hydrodynamics basics
  • UltraScan LIMS
  • Analytes, Buffers, Solutions
  • Data Acquisition and setting up an experiment
  • Manual LIMS data import
  • Interference, absorbance, multi-wavelength data editing
Session 3: Tuesday, 8/22/23 - Experimental Design
  • Cells, Centerpieces, Rotors, Optics, loading volume, # of scans
  • Rotor Speed and Sed. Velocity Simulation
  • Sed. Equilibrium vs. Sed. Velocity
  • What AUC can and cannot tell you
  • How to optimize your experimental design
  • Analysis Strategies
Session 4: Tuesday, 8/22/23 - Sed. Veloc. Analysis I
  • Understanding Noise in Data
  • Finite Element Modeling Basics
  • Model Dependent vs. Model Independent Analysis
  • Sed. Veloc. Analysis Workflow
  • Boundary Condition fitting
  • Reversible interactions
  • 2-dimensional Spectrum Analysis
Session 5: Wednesday, 8/23/23 - Sed. Veloc. Analysis II
  • Parametrically Constrained Spectrum Analysis
  • Genetic Algorithms
  • Monte Carlo analysis
  • van Holde - Weischet Analysis
  • Application examples:
    • Aggregation
    • Hetero-Interactions
    • Molar Mass, Size and Shape analysis
    • Concentration dependent non-ideality
Session 6: Wednesday, 8/23/23 - Advanced Concepts I
  • Global Analysis
  • Application examples:
    • monoclonal antibodies
  • Discrete Model Genetic Algorithms
  • Application examples:
    • Reversible Association
  • Custom Grid Analysis and Applications
  • Partial Specific Volume Distributions and Density
Session 7: Thursday, 8/24/23 - Advanced Concepts II
  • D2O density matching
  • Application examples:
    • Vaccines and Lipid Nanoparticles
  • UV/vis spectral analysis
  • Concepts in multi-wavelength AUC experiments
  • Application examples:
    • Hetero-Interactions
    • Protein-Nucleic Acid Interactions
    • AAV
Session 8: Thursday, 8/24/23 - GMP analysis
  • Instrument Calibrations
  • Development of GMP protocol
  • GMP data acquisition
  • Reporting
  • Electronic signatures and audit trails
Session 9: Friday, 8/25/23 - UltraScan SOMO
Presented by Dr. Emre Brookes
Registration Deadline:
July 15th, 2023

Register here now
Additional Information:

Workshop Materials | Contacts | Accommodations | Parking Info | Travel to Lethbridge | Registration | Directions